Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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ERR2275801

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Mosquito RNA-seq with B grade (83/100) substantially lowered by catastrophic duplication bias (79.67%, scored 0/100), the sole metric that scored zero and overwhelming all else. Exceptional per-base quality (Q30 96.9%, mean 38) is negated by duplication, likely from PCR over-amplification. Unsuitable for expression quantification without computational deduplication.

Data type / assay
bulk-RNA-seq
Organism
Anopheles coluzzii
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 1006386774 reported
total reads 19733074 reported
n content pct 0.01 measured
pct q20 bases 99 measured
pct q30 bases 96.9 measured
gc content pct 55.5 measured
mean read length 51 measured
mean base quality 38 measured
adapter content pct 0 measured
duplication rate pct 79.67 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.9 measured ×1 100%
mean base quality 38 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 79.67 measured ×0.4 0%
QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0