ERR2357662

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

84/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Honeybee ncRNA-seq with B grade (84/100) substantially limited by high duplication (72.66%, scored 5/100, second highest among RNA datasets), reflecting PCR stress typical in small RNA libraries. Good Q30 (93.2%) and high read count (45.8M) are offset by duplication. Suitable for abundance surveys but not quantitative expression estimates.

Data type / assay

bulk-RNA-seq

Organism

Apis mellifera

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 3041949669 reported

total reads 45785408 reported

n content pct 0.003 measured

pct q20 bases 95.1 measured

pct q30 bases 93.2 measured

gc content pct 53.7 measured

mean read length 34.4 measured

mean base quality 34.3 measured

adapter content pct 0 measured

duplication rate pct 72.66 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 84/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.2 measured ×1 100%

mean base quality 34.3 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 72.66 measured ×0.4 5%

QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0