ERR2360692

ENA first seen 2014

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

65/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Rat gut metagenome amplicon (MiSeq). Grade-D failure (65/100) with catastrophic duplication (96.33%) indicating collapse to near-clonal amplification and severe PCR bias, measured. Base quality (Q30=79.5%, scored 48/100) is also below threshold. Unusable for reliable taxonomic profiling; immediate re-sequencing required.

Data type / assay

amplicon

Organism

rat gut metagenome

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 149311717 reported

total reads 248679 reported

n content pct 0 measured

pct q20 bases 89.9 measured

pct q30 bases 79.5 measured

gc content pct 52.1 measured

mean read length 299.6 measured

mean base quality 33.1 measured

adapter content pct 0.07 measured

duplication rate pct 96.33 measured

How this grade was computed

Weighted mean of 2 scored metric(s) → 65/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 79.5 measured ×1 48%

adapter content pct 0.07 measured ×0.5 100%

QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0