ERR2803690

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

65/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human amplicon. Marginal reusability (grade D) with borderline Q30 (80.4%, scored 52/100) and elevated N-content (0.754%). Critical limitation is extrapolated coverage (0.4x), which is below practical thresholds; deep measured validation is pending before reuse can be recommended.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 23635056 reported

total reads 44394 reported

n content pct 0.754 measured

pct q20 bases 88.7 measured

pct q30 bases 80.4 measured

gc content pct 52.6 measured

mean read length 250.1 measured

mean base quality 33.7 measured

adapter content pct 2.11 measured

duplication rate pct 80.87 measured

mean target coverage 0.4 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 65/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.4 measured ×1 52%

adapter content pct 2.11 measured ×0.5 92%

QC cost 4 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0