ERR2804193

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

29/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Failed amplicon (F grade, 29/100) with critically low Q30 (69.9%, scored 0/100, worst in cohort) and essentially no coverage (1,336 total reads, 0x mean_target_coverage, extrapolated). Suggests complete sequencing or amplification failure. Do not reuse under any circumstances.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 670672 reported

total reads 1336 reported

n content pct 0.091 measured

pct q20 bases 83.9 measured

pct q30 bases 69.9 measured

gc content pct 52.1 measured

mean read length 251 measured

mean base quality 29.5 measured

adapter content pct 2.99 measured

duplication rate pct 61 measured

mean target coverage 0 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 29/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 69.9 measured ×1 0%

adapter content pct 2.99 measured ×0.5 86%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0