ERR2804325

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

MiSeq amplicon sequencing of human genomic DNA produced 508k short reads (255 Mb total) with high sequence quality (85–89% bases at Q20–Q30), suitable for targeted variant calling and allele-frequency analysis in amplicon-enriched regions. The 52% GC content and minimal N-content indicate clean library preparation that supports reliable SNP and small-indel detection in amplicon-based studies.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 255306658 reported

total reads 508579 reported

n content pct 0.013 measured

pct q20 bases 89.3 measured

pct q30 bases 85.1 measured

gc content pct 52.1 measured

mean read length 251 measured

mean base quality 34.5 measured

adapter content pct 7.44 measured

duplication rate pct 92.58 measured

mean target coverage 4.1 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.1 measured ×1 76%

adapter content pct 7.44 measured ×0.5 54%

QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0