Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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ERR2804390

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human amplicon on MiSeq with moderate-to-low quality (88.5% Q20, 81.9% Q30) and elevated N-content (0.722%). High ambiguous-base frequency indicates potential PCR stalling or template secondary structure; caution warranted for allele discrimination and population inference.

Data type / assay
amplicon
Organism
Homo sapiens
Instrument
Illumina MiSeq
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 221595534 reported
total reads 442366 reported
n content pct 0.722 measured
pct q20 bases 88.5 measured
pct q30 bases 81.9 measured
gc content pct 52.2 measured
mean read length 250.4 measured
mean base quality 34.5 measured
adapter content pct 3.61 measured
duplication rate pct 91.74 measured
mean target coverage 3.6 extrapolated
How this grade was computed
Weighted mean of 2 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 81.9 measured ×1 60%
adapter content pct 3.61 measured ×0.5 81%
QC cost 34 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0