ERR2804402

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

66/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Human amplicon sequencing on MiSeq showing moderate quality (87.9% Q20, 80.7% Q30) with notably high N-content (0.732%). Elevated ambiguous base calls warrant careful interpretation in genotyping or phylogenetic applications; suggests possible contamination or sequencing issues during library preparation.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

Illumina MiSeq

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 163442656 reported

total reads 326388 reported

n content pct 0.732 measured

pct q20 bases 87.9 measured

pct q30 bases 80.7 measured

gc content pct 52.5 measured

mean read length 250.4 measured

mean base quality 34.3 measured

adapter content pct 2.09 measured

duplication rate pct 90.26 measured

mean target coverage 2.6 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 66/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 80.7 measured ×1 54%

adapter content pct 2.09 measured ×0.5 92%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0