ERR3025163

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk-RNA-seq on HiSeq 2500 for Drosophila simulans (closely related to D. melanogaster) with high quality (97.9% Q20, 95.8% Q30) and slightly elevated N-content (0.304%). Enables comparative transcriptomics between sibling Drosophila species and supports studies of regulatory divergence and hybrid viability.

Data type / assay

bulk-RNA-seq

Organism

Drosophila simulans

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 856232966 reported

total reads 16942492 reported

n content pct 0.304 measured

pct q20 bases 97.9 measured

pct q30 bases 95.8 measured

gc content pct 49.5 measured

mean read length 50.5 measured

mean base quality 35.8 measured

adapter content pct 0.01 measured

duplication rate pct 34.72 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.8 measured ×1 100%

mean base quality 35.8 measured ×0.6 100%

adapter content pct 0.01 measured ×0.4 100%

duplication rate pct 34.72 measured ×0.4 90%

QC cost 36 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0