ERR3150556

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

92/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Bulk-RNA-seq on HiSeq 2500 for Anopheles gambiae with high quality (97.9% Q20, 95.4% Q30). Additional A. gambiae replicate enables multi-condition transcriptome studies addressing vector immune responses, insecticide-resistance mechanisms, or developmental transitions.

Data type / assay

bulk-RNA-seq

Organism

Anopheles gambiae

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), BAM/CRAM (aligned)

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 682215300 reported

total reads 9096204 reported

n content pct 0.007 measured

pct q20 bases 97.9 measured

pct q30 bases 95.4 measured

gc content pct 52.4 measured

mean read length 75 measured

mean base quality 35.8 measured

adapter content pct 0.1 measured

duplication rate pct 50.71 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 92/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.4 measured ×1 100%

mean base quality 35.8 measured ×0.6 100%

adapter content pct 0.1 measured ×0.4 100%

duplication rate pct 50.71 measured ×0.4 54%

QC cost 35 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0