ERR3358311

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

NextSeq 500 bulk RNA-seq from the fungus-farming ant Acromyrmex echinatior (10.3M reads, 774.8 Mb, 63.4% GC) with good quality (94.8% Q20, 93.2% Q30, 0.001% N). Enables gene expression studies in social insect biology and ant-fungus symbiosis. Ant transcriptomics and NextSeq insect sequencing searches would retrieve this dataset.

Data type / assay

bulk-RNA-seq

Organism

Acromyrmex echinatior

Instrument

NextSeq 500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 774798900 reported

total reads 10330652 reported

n content pct 0.001 measured

pct q20 bases 94.8 measured

pct q30 bases 93.2 measured

gc content pct 63.4 measured

mean read length 75 measured

mean base quality 34.2 measured

adapter content pct 10.5 measured

duplication rate pct 65.28 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.2 measured ×1 100%

mean base quality 34.2 measured ×0.6 100%

adapter content pct 10.5 measured ×0.4 53%

duplication rate pct 65.28 measured ×0.4 22%

QC cost 29 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0