ERR4026990

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

98/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

NextSeq 550 bulk RNA-seq from the dengue/yellow-fever vector Aedes aegypti (34.5M reads, 5.2 Gb, 48.7% GC) with premium quality (98.2% Q20, 97.3% Q30, 0.005% N). High-quality transcriptomic dataset enables comprehensive gene expression profiling for arbovirus vector biology. Mosquito transcriptomics and NextSeq short-read sequencing searches would locate this.

Data type / assay

bulk-RNA-seq

Organism

Aedes aegypti

Instrument

NextSeq 550

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 5207950436 reported

total reads 34507739 reported

n content pct 0.005 measured

pct q20 bases 98.2 measured

pct q30 bases 97.3 measured

gc content pct 48.7 measured

mean read length 75.5 measured

mean base quality 35.1 measured

adapter content pct 0 measured

duplication rate pct 35.52 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 98/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.3 measured ×1 100%

mean base quality 35.1 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 35.52 measured ×0.4 88%

QC cost 57 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0