Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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ERR4676859

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

78/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

NovaSeq 6000 RNA-seq of the butterfly Leptidea sinapis generated 28M reads (4.2 Gb) with exceptional quality (96–99% bases at Q20–Q30) and minimal sequencing error, enabling sensitive detection of developmentally-regulated transcripts and host-microbe interactions in this lepidopteran species. High-depth transcriptomics and insect developmental biology searches apply.

Data type / assay
bulk-RNA-seq
Organism
Leptidea sinapis
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 4242273124 reported
total reads 28094524 reported
n content pct 0.001 measured
pct q20 bases 98.8 measured
pct q30 bases 96.6 measured
gc content pct 48.1 measured
mean read length 151 measured
mean base quality 36.4 measured
adapter content pct 10.35 measured
duplication rate pct 68.58 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 78/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.6 measured ×1 100%
mean base quality 36.4 measured ×0.6 100%
adapter content pct 10.35 measured ×0.4 54%
duplication rate pct 68.58 measured ×0.4 14%
QC cost 20 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0