Corpus 1,280 assessed · 1,181 scored · 646 reproduced ≥75 · 170 flagged ·∅ 74/100
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ERR4676865

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

NovaSeq 6000 RNA-seq of Leptidea sinapis generated 23.8M reads (3.5 Gb, 48.5% GC) with excellent quality (95–98% bases at Q20–Q30), enabling accurate quantification of transcript abundances and confident detection of lowly-expressed regulatory genes in butterfly developmental and tissue-specific studies.

Data type / assay
bulk-RNA-seq
Organism
Leptidea sinapis
Instrument
Illumina NovaSeq 6000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads), submitted files
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 3594368062 reported
total reads 23803762 reported
n content pct 0 measured
pct q20 bases 98.3 measured
pct q30 bases 95.9 measured
gc content pct 48.5 measured
mean read length 151 measured
mean base quality 36.3 measured
adapter content pct 10.49 measured
duplication rate pct 65.78 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 95.9 measured ×1 100%
mean base quality 36.3 measured ×0.6 100%
adapter content pct 10.49 measured ×0.4 53%
duplication rate pct 65.78 measured ×0.4 21%
QC cost 22 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0