ERR489303

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Illumina HiSeq 2000 bulk RNA-seq from Anopheles gambiae (206.3M reads, 41.7 Gb, 52.5% GC) with very high depth but lower quality (91.7% Q20, 83.3% Q30, 0.214% N). Exceptional read accumulation enables discovery of rare transcripts and novel isoforms. Reuse suits abundance-based analyses; variant calling requires filtering.

Data type / assay

bulk-RNA-seq

Organism

Anopheles gambiae

Instrument

Illumina HiSeq 2000

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 41674647876 reported

total reads 206310138 reported

n content pct 0.214 measured

pct q20 bases 91.7 measured

pct q30 bases 83.3 measured

gc content pct 52.5 measured

mean read length 101 measured

mean base quality 33.1 measured

adapter content pct 0.1 measured

duplication rate pct 36.31 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.3 measured ×1 67%

mean base quality 33.1 measured ×0.6 85%

adapter content pct 0.1 measured ×0.4 100%

duplication rate pct 36.31 measured ×0.4 86%

QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0