ERR843877

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

78/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

HiSeq 2500 miRNA-seq of the aphid Acyrthosiphon pisum produced 5M reads (250 Mb, 51.4% GC) with high quality (86–92% bases at Q20–Q30), supporting microRNA abundance profiling and discovery of regulatory RNAs in this model pest insect. Aphid miRNA, plant-insect interactions, and invertebrate ncRNA searches apply to this dataset.

Data type / assay

bulk-RNA-seq

Organism

Acyrthosiphon pisum

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 250481850 reported

total reads 5009637 reported

n content pct 0.007 measured

pct q20 bases 91.7 measured

pct q30 bases 86.4 measured

gc content pct 51.4 measured

mean read length 50 measured

mean base quality 34.5 measured

adapter content pct 0 measured

duplication rate pct 68.47 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 78/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 86.4 measured ×1 82%

mean base quality 34.5 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 68.47 measured ×0.4 14%

QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0