ERR843901

ENA first seen 2021

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

68/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

miRNA-seq (Acyrthosiphon pisum). Grade D, 68/100. Duplication catastrophically high at 62.45% (measured) severely limits power for differential calls; Q30 at 82.2% marginal for small RNA work. Reuse not recommended.

Data type / assay

bulk-RNA-seq

Organism

Acyrthosiphon pisum

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 300000000 reported

total reads 6000000 reported

n content pct 0.013 measured

pct q20 bases 89 measured

pct q30 bases 82.2 measured

gc content pct 52.4 measured

mean read length 50 measured

mean base quality 33.2 measured

adapter content pct 0 measured

duplication rate pct 62.45 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 68/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 82.2 measured ×1 61%

mean base quality 33.2 measured ×0.6 87%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 62.45 measured ×0.4 28%

QC cost 55 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0