Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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ERR852099

ENA first seen 2018

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

2 further papers cite this accession but reuse could not be confirmed.

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:opus

This is a small-RNA sequencing library (miRNA-Seq on Illumina HiSeq 2000, human), correctly reflected in the ~24 bp mean read length that is characteristic of mature microRNAs. In QC terms it is a solid but not pristine dataset: base quality is excellent (Q30 at 96.3%, mean base quality 38.3, and effectively zero adapter and N content), which is what holds the score at a B and makes the per-base calls trustworthy for reuse. The grade was dragged down almost entirely by an 84.71% duplication rate, which scored 0 and is the single biggest detractor — though for a miRNA library this is partly expected, since a small set of highly abundant microRNAs is resequenced many times, so it reflects library complexity limits more than outright failure and chiefly caps sensitivity for lowly expressed species. With evidence_strength at 1 the quality metrics here were genuinely measured rather than extrapolated, so this reading is reliable rather than provisional; just note that total_reads and total_bases are reported values, so confirm depth independently if your analysis is coverage-sensitive.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
N numbers (samples, groups)
1 runs
Metrics (value · how obtained)
checksum ok yes reported
total bases 388270276 reported
total reads 15945439 reported
n content pct 0 measured
pct q20 bases 99 measured
pct q30 bases 96.3 measured
gc content pct 52.5 measured
mean read length 24.3 measured
mean base quality 38.3 measured
adapter content pct 0 measured
duplication rate pct 84.71 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.3 measured ×1 100%
mean base quality 38.3 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 84.71 measured ×0.4 0%
QC cost 17 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 89