ERR973611

This is a human miRNA-Seq library (small-RNA fraction, single-end 35 bp reads on a HiSeq 1000), and it earns a solid B (83/100): base quality is excellent, with 96.6% of bases at Q30 and a mean base quality of 37.6, plus zero adapter and N content, so the reads themselves are clean and confidently called. The grade is held back almost entirely by a 91.36% duplication rate, which scored 0 and is the single dominant drag on the score; while very high duplication is expected and largely benign in miRNA-Seq (a small number of mature miRNA species are sequenced very deeply), it does mean the ~47M reads collapse to far fewer unique molecules, limiting effective library complexity and the detection of low-abundance miRNAs. For reuse this dataset is trustworthy for profiling and quantifying moderately-to-highly expressed miRNAs, but treat low-count features and any novel/rare-species claims cautiously. Note that evidence_strength is low (1) because the headline volume figures (total_reads, total_bases, checksum) are reported rather than independently measured, so this reading is provisional pending a full measured QC pass.