ERX3622297

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

48/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Mus musculus amplicon sequencing via 454 GS FLX Titanium yielding 8.6 million bases across 27.8K reads—older pyrosequencing platform with lower per-read quality (91.3% Q20, 74.4% Q30) and susceptibility to homopolymer run errors. Limited read count and 52.7% GC restrict assembly complexity. Not recommended for new projects; suitable only for targeted historical data integration.

Data type / assay

amplicon

Organism

Mus musculus

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 8663191 reported

total reads 27836 reported

n content pct 0.04 measured

pct q20 bases 91.3 measured

pct q30 bases 74.4 measured

gc content pct 52.7 measured

mean read length 311.2 measured

mean base quality 33.6 measured

adapter content pct 0 measured

duplication rate pct 90.22 measured

mean target coverage 0.2 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 48/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 74.4 measured ×1 22%

adapter content pct 0 measured ×0.5 100%

QC cost 1 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0