ERX3622402

ENA first seen 2019

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

79/100 · C

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Amplicon sequencing with marginal technical quality and critically low target coverage. Q30 bases (83.6%) and 454 platform limitations constrain accuracy; mean target coverage of only 0.1× (extrapolated) indicates severe amplicon failure or technical malfunction. Not recommended for reuse without troubleshooting the underlying amplicon assay.

Data type / assay

amplicon

Organism

Homo sapiens

Instrument

454 GS FLX Titanium

Platform

LS454

Files available

FASTQ (raw reads), submitted files

N numbers (samples, groups)

1 runs

Metrics (value · how obtained)

checksum ok yes reported

total bases 7684601 reported

total reads 19201 reported

n content pct 0 measured

pct q20 bases 95 measured

pct q30 bases 83.6 measured

gc content pct 53.8 measured

mean read length 400.2 measured

mean base quality 35.7 measured

adapter content pct 0 measured

duplication rate pct 96.06 measured

mean target coverage 0.1 extrapolated

How this grade was computed

Weighted mean of 2 scored metric(s) → 79/100

The C grade is a transparent weighted average. Each metric below scored from 0–100% against the published amplicon thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 83.6 measured ×1 68%

adapter content pct 0 measured ×0.5 100%

QC cost 16 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0