Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE100007

GEO first seen 2017

Widespread translational remodeling during human neuronal differentiation

Organism
Homo sapiens
Samples
52
Type
Expression profiling by high...
Submitted
2017-06-13

Faithful cellular differentiation requires precise coordination of changes in gene expression. However, the relative contributions of transcriptional and translational regulation during human cellular differentiation are unclear. Here, we induced forebrain neuronal differentiation of human embryonic stem cells (hESCs) and characterized genome-wide RNA and translation levels during neurogenesis. We find that thousands of genes change at the translation level across differentiation without a corre...

Provenance — who produced it, who reused it

Linked to 5 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
John D BlairDirk HockemeyerJennifer A DoudnaHelen S BateupStephen N Floor
Reused by

3 further papers cite this accession but reuse could not be confirmed.

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq from Homo sapiens; A-grade (95/100). Elevated duplication at 42.65% and minor adapters (2.27%) limit reliability; measured metrics confirm high base quality (Q39.9 mean), but duplication is the principal reuse concern requiring robust normalization to prevent inflated effect-size estimates.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.026 measured
pct q20 bases 99.2 measured
pct q30 bases 97.6 measured
gc content pct 54.2 measured
mean read length 101 measured
mean base quality 39.9 measured
adapter content pct 2.27 measured
duplication rate pct 42.65 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97.6 measured ×1 100%
mean base quality 39.9 measured ×0.6 100%
adapter content pct 2.27 measured ×0.4 99%
duplication rate pct 42.65 measured ×0.4 72%
QC cost 21 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0

Scientific quality

Based on hands-on reproduction of the papers that use this dataset. A reproducible paper that stands on this data is positive evidence; a flagged one is a prompt to look closer — never a verdict on the dataset itself without the evidence.

1 studies use it 1 reproduced mean score 90