Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
← Dataset search

GSE100483

GEO first seen 2018

RNA-seq of MDA-MB-231 cells with TET1 knockout

Organism
Homo sapiens
Samples
12
Type
Expression profiling by high...
Submitted
2017-06-26

DNA hypermethylation is known to contribute to the formation of cancer. However, DNA hypomethylation has received far less attention and the factors controlling the balance between hypo and hypermethylation and its impact on tumorigenesis remains unclear. Triple negative breast cancer (TNBC), a subtype of breast cancer that does not overexpress the hormone receptors or HER2/NEU, is one of the most hypomethylated cancers observed. Importantly, TNBCs are often a therapeutic challenge because of...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Charly R GoodShoghag PanjarianAndrew D KellyJozef MadzoBela PatelJaroslav JelinekJean-Pierre Issa

Deep data QC

87/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human bulk RNA-seq with exceptionally high measured quality (99% Q20, 98% Q30) across 3.3k samples at 50 bp mean read length, but metadata unavailable (HTTP fetch error). Measured Q-scores are extraordinary; experimental design and biological context essential for determining suitability for reuse.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.007 measured
pct q20 bases 99 measured
pct q30 bases 98 measured
gc content pct 46.8 measured
mean read length 50 measured
mean base quality 38.9 measured
adapter content pct 0 measured
duplication rate pct 64.08 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 87/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98 measured ×1 100%
mean base quality 38.9 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 64.08 measured ×0.4 24%
QC cost 35 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0