Transcriptomics analysis of gene expression in control, Prrc2a conditional knockout, and Fto transgene mouse brain sample
RNA was isolated from hippocampus sample from 4 weeks old prrc2a flox/flox and prrc2a flox/flox;Olig2-cre or 4 weeks old wt and Fto Tg micemice using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) of higher than 9. Approximately 2.5 µg of total RNA was then used for library preparation using a TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, US...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deep data QC
94/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Mouse (Mus musculus) bulk RNA-seq with exceptionally high base quality (98.7% Q20, 96.7% Q30, 101 bp reads, mean quality 39.5) and minimal adapter contamination (0.24%) despite GEO metadata unavailability. Excellent metrics indicate optimal library preparation and sequencing. Gene expression profiling and isoform discovery are well-supported; full experimental context requires contact with data submitters.
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0