Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE100623

GEO first seen 2019

SET-9 and SET-26, the C. elegans homologs of human MLL5, are critical for germline development and longevity

Organism
Caenorhabditis elegans
Samples
24
Type
Expression profiling by high...
Submitted
2017-06-28

SET-9 and SET-26 are highly homologous paralogs that share redundant function in germline development, but SET-26 alone plays a key role in longevity and heat stress response. SET-26 is broadly expressed, but SET-9 is only detectable in the germline, which likely account for their different biological roles. SET-9 and SET-26 bind to H3K4me3 in vitro and in vivo, and their loss results in broadening of H3K4me3 domains. SET-9 and SET-26 also regulate the RNA expression of a subset of their target...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Wenke Wang
Reused by

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Caenorhabditis elegans
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
24 / 24 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 84201610437 reported
total reads 950197637 reported
n content pct 0.029 measured
pct q20 bases 95 measured
pct q30 bases 90.8 measured
gc content pct 47.1 measured
mean read length 101 measured
mean base quality 35 measured
adapter content pct 0.9 measured
duplication rate pct 35.24 measured
supplementary file types BEDGRAPH, TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.8 measured ×1 100%
QC cost 18 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0