Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE100955

GEO first seen 2021

Genome-wide maps of Tet1 binding in [WT, Tet1-Flag] and [Dnmt3a-/-, Tet1-Flag] J1 ES cells

Organism
Mus musculus
Samples
6
Type
Genome binding/occupancy pro...
Submitted
2017-07-08

In order to assess Tet1 binding, we first generated a Flag tagged Tet1 ES cells and then knocked out Dnmt3a in the [WT, Tet1-Flag] cells. By Tet1 ChIP and Flag ChIP, we showed that Tet1 binding was complementary to Dnmt3a. And Tet1 binding was not affected or slightly increased at majority of its targets.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Xueqiu LinTianpeng GuWei LiMargaret A Goodell
Reused by

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.042 measured
pct q20 bases 88.1 measured
pct q30 bases 84.1 measured
gc content pct 43.3 measured
mean read length 74.6 measured
mean base quality 32.2 measured
adapter content pct 0.04 measured
duplication rate pct 5.83 measured
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 84.1 measured ×1 71%
QC cost 47 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0