Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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GSE100971

GEO first seen 2017

Next Generation Sequencing of total RNA from 9 bladder cancer cell lines and 3 fractionated bladder cancer cell lines

Organism
Homo sapiens
Samples
21
Type
Non-coding RNA profiling by...
Submitted
2017-07-09

We profile the cell line expression of 279 circRNAs, that are highly expressed across 457 bladder cancer patient samples. Additionally, we investigate their cellular location in fractionated cell lines

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Trine H OkholmLars D AndersenLise ChristensenMorten M NielsenJakob S PedersenSøren VangJakob Hedegaard
Reused by

Deep data QC

95/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human bulk RNA-seq with high base quality (96.4% Q20, 90.4% Q30, 101 bp reads, mean quality 35.3) and 6.48% adapter content despite GEO metadata unavailability. Excellent base metrics indicate high-quality library preparation. Gene expression profiling and isoform discovery are well-supported; full experimental context requires contact with data submitters or GEO curators.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.01 measured
pct q20 bases 96.4 measured
pct q30 bases 90.4 measured
gc content pct 48.6 measured
mean read length 101 measured
mean base quality 35.3 measured
adapter content pct 6.48 measured
duplication rate pct 32.6 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 95/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.4 measured ×1 100%
mean base quality 35.3 measured ×0.6 100%
adapter content pct 6.48 measured ×0.4 75%
duplication rate pct 32.6 measured ×0.4 94%
QC cost 41 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0