Corpus 1,283 assessed · 1,184 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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GSE101112

GEO first seen 2019

Whole transcriptome analysis of brain hippocampal tissue from SAMP8 mice and rat primary neurons treated with the Alzheimer's disease drug candidates CMS121 and J147

Organism
Mus musculus; Rattus norvegicus
Samples
32
Type
Expression profiling by high...
Submitted
2017-07-10

Alzheimer's disease (AD) drug discovery has rarely been addressed in the context of aging even though sporadic AD accounts for 99% of the cases. Phenotypic screens based upon old age-associated brain toxicities were used to develop the potent AD drug candidates CMS121 and J147. The aim of this project was to investigate whether these two different AD drug candidates prevented the progression of dementia in SAMP8 mice when administered at advanced stages of disease, and whether they shared common...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Antonio CurraisLing Huang
Reused by

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Multi-species bulk RNA-seq (Mus musculus and Rattus norvegicus) via HiSeq 2500 with 30.9 billion bases across 607 million reads from 32 FASTQ files—enables cross-species comparative transcriptomics and identification of conserved gene regulatory networks. Exceptional quality (98.1% Q20, 96.1% Q30) supports robust expression quantification. Suitable for mammalian evolution and disease-model comparison.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus; Rattus norvegicus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
32 / 32 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 30959143581 reported
total reads 607042031 reported
n content pct 0.001 measured
pct q20 bases 98.1 measured
pct q30 bases 96.1 measured
gc content pct 46.9 measured
mean read length 51 measured
mean base quality 35.9 measured
adapter content pct 0.07 measured
duplication rate pct 24.33 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.1 measured ×1 100%
mean base quality 35.9 measured ×0.6 100%
adapter content pct 0.07 measured ×0.4 100%
duplication rate pct 24.33 measured ×0.4 100%
QC cost 13 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0