Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE101714

GEO first seen 2022

A TAD boundary at the HoxD locus segregates opposing limb regulatory landscapes and their target genes [ChIP-Seq]

Organism
Mus musculus
Samples
13
Type
Genome binding/occupancy pro...
Submitted
2017-07-20

The mammalian HoxD cluster is positioned at the boundary between two topologically associating domains (TADs), each of them matching a distinct, enhancer-rich regulatory landscape. During limb development, the telomeric TAD controls the early phase of Hoxd gene transcription in future forearm cells, whereas the centromeric TAD subsequently regulates transcription of more posterior Hoxd genes in presumptive digit cells. The TAD boundary is essential as it prevents the terminal Hoxd13 gene to resp...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
E Rodriguez-CarballoL Lopez-DelisleL BeccariD Duboule
Reused by

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.033 measured
pct q20 bases 99 measured
pct q30 bases 96.7 measured
gc content pct 41.7 measured
mean read length 50 measured
mean base quality 39.3 measured
adapter content pct 0.01 measured
duplication rate pct 12.17 measured
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.7 measured ×1 100%
QC cost 33 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0