Corpus 1,283 assessed · 1,184 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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GSE101797

GEO first seen 2017

RNA-seq of regenerating blastema cells in Drosophila melanogaster

Organism
Drosophila melanogaster
Samples
6
Type
Expression profiling by high...
Submitted
2017-07-24

We have carried out eukaryotic whole-genome Illumina RNA-seq of regenerating blastema cells and control undamaged wing imaginal disc cells to identify the differentially expressed genes during regeneration.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Sumbul J KhanRachel Smith-Bolton
Reused by

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Drosophila melanogaster bulk RNA-seq via HiSeq 2000 spanning ~17.8 billion bases across 178 million reads with 96.7% Q20 bases—suited for genome-wide gene expression profiling, isoform discovery, and developmental transcriptomics. High Q30 (90.9%) and minimal N-content (0.022%) support splice junction detection and recovery of low-abundance transcripts in this model organism.

Data type / assay
bulk-RNA-seq
Organism
Drosophila melanogaster
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
6 / 6 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 17821864300 reported
total reads 178218643 reported
n content pct 0.022 measured
pct q20 bases 96.7 measured
pct q30 bases 90.9 measured
gc content pct 48.7 measured
mean read length 100 measured
mean base quality 35.3 measured
adapter content pct 0.62 measured
duplication rate pct 30.36 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.9 measured ×1 100%
mean base quality 35.3 measured ×0.6 100%
adapter content pct 0.62 measured ×0.4 100%
duplication rate pct 30.36 measured ×0.4 99%
QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0