Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE102271

GEO first seen 2018

Expression analysis of melanocyte stem cells from Mitfmi-vga9/+ mice reveals a role for MITF in the transcriptional regulation of innate immune gene expression

Organism
Mus musculus
Samples
6
Type
Expression profiling by high...
Submitted
2017-08-04

Melanocyte stem cells (McSCs) and mouse models of hair graying serve as useful systems to uncover mechanisms involved in stem cell self-renewal and the maintenance of regenerating tissues. Interested in assessing genetic variants of hair graying, we found that the Mitfmi-vga9/+ strain of mice is susceptible to loss of McSC maintenance via ectopic McSC differentiation. Based on transcriptome and molecular analyses of Mitfmi-vga9/+ mice we report a novel role for MITF in the regulation of systemic...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Melissa L HarrisTemesgen D FufaSandeep S JoshiDenise M LarsonArturo IncaoDerek E GildeaNiraj S TrivediJoseph W PalmerAutumne N LeeThomas J HornyakWilliam J Pavan
Reused by

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (Mus musculus). Grade A, 100/100. Q30 bases 92.8% and mean base quality 36.1 (both measured) are excellent; duplication 27.16% acceptable for quantification. Ready for reuse.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
12 / 6 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 70926172936 reported
total reads 351119668 reported
n content pct 0 measured
pct q20 bases 98.1 measured
pct q30 bases 92.8 measured
gc content pct 48.6 measured
mean read length 101 measured
mean base quality 36.1 measured
adapter content pct 0.05 measured
duplication rate pct 27.16 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 92.8 measured ×1 100%
mean base quality 36.1 measured ×0.6 100%
adapter content pct 0.05 measured ×0.4 100%
duplication rate pct 27.16 measured ×0.4 100%
QC cost 28 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0