Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE102315

GEO first seen 2018

Nutritional control of protein translation

Organism
Homo sapiens
Samples
20
Type
Other; Expression profiling...
Submitted
2017-08-07

Regulation of protein translation is a key feature of many biological processes. Global protein translation as well as translation at the codon level can be regulated by RNA modifications. These modifications are particularly enriched in tRNAs, where they represent an additional regulatory layer on top of the primary RNA sequence. In eukaryotes, levels of tRNA queuosinylation reflect the bioavailability of the precursor queuine, which is salvaged from the gut microbiota and absorbed in the intes...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Francesca TuortoCarine Legrand
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

67/100 · D

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
20 / 20 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 29733241179 reported
total reads 583004729 reported
n content pct 0.003 measured
pct q20 bases 99.1 measured
pct q30 bases 98 measured
gc content pct 58 measured
mean read length 51 measured
mean base quality 37.1 measured
adapter content pct 94.07 measured
duplication rate pct 73.68 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 67/100

The D grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 98 measured ×1 100%
mean base quality 37.1 measured ×0.6 100%
adapter content pct 94.07 measured ×0.4 0%
duplication rate pct 73.68 measured ×0.4 3%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0