Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE102317

GEO first seen 2017

STAT5-mediated chromatin interactions in superenhancers activate IL-2 highly inducible genes: Functional dissection of the Il2ra gene locus

Organism
Mus musculus
Samples
28
Type
Expression profiling by high...
Submitted
2017-08-07

Cytokines critically control immune responses, but how regulatory programs are altered to allow T cells to differentially respond to distinct cytokine stimuli remains poorly understood. Here, we have globally analyzed enhancer elements bound by IL-2-activated STAT5 and IL-21-activated STAT3 in T cells and identified Il2ra as the top-ranked gene regulated by an IL-2-activated STAT5-bound superenhancer and one of the top genes regulated by STAT3-bound superenhancers. Moreover, we found that STAT5...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Peng LiSuman MitraRosanne SpolskiJangsuk OhWei LiaoZhonghui TangFei MoXingwang LiErin E WestDaniel GromerJian-Xin LinChengyu LiuYijun RuanWarren J Leonard
Reused by

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Instrument
Illumina Genome Analyzer II
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
28 / 28 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 37518914548 reported
total reads 806412731 reported
n content pct 0.005 measured
pct q20 bases 96.4 measured
pct q30 bases 91.6 measured
gc content pct 48.6 measured
mean read length 50 measured
mean base quality 36 measured
adapter content pct 0.02 measured
duplication rate pct 18.27 measured
supplementary file types BEDGRAPH, TXT reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 91.6 measured ×1 100%
QC cost 19 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0