Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE102334

GEO first seen 2017

RNA-Sequencing of EC and PVC from aged APOE mice

Organism
Mus musculus
Samples
58
Type
Expression profiling by high...
Submitted
2017-08-07

RNA-Sequencing of entorhinal cortex and primary visual cortex from 14-15 month old APOE3/4 vs. APOE3/3 targeted replacement mice.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Tal NurielAllissa A DillmanMark R CooksonKaren E Duff
Reused by

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Large-scale mouse (Mus musculus) bulk RNA-seq on HiSeq 2000 comprising 1.97 billion reads across 66 FASTQ files, with 95.3% Q20 and 87% Q30 base quality. Sequencing depth is sufficient for detailed gene expression studies, tissue-specific isoform detection, and comparative transcriptomics. Slightly lower Q30 (87%) suggests value in screening for 3′ polyadenylation bias or adapter artifacts.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
66 / 58 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 395411955600 reported
total reads 1977059778 reported
n content pct 0.042 measured
pct q20 bases 95.3 measured
pct q30 bases 87 measured
gc content pct 49.5 measured
mean read length 100 measured
mean base quality 34.5 measured
adapter content pct 0.22 measured
duplication rate pct 7.11 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 87 measured ×1 85%
mean base quality 34.5 measured ×0.6 100%
adapter content pct 0.22 measured ×0.4 100%
duplication rate pct 7.11 measured ×0.4 100%
QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0