Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE102357

GEO first seen 2019

hnRNPC regulates cancer-specific alternative cleavage and polyadenylation profiles

Organism
Homo sapiens
Samples
32
Type
Expression profiling by high...
Submitted
2017-08-08

Alternative cleavage and polyadenylation (APA) can occur at more than half of all human genes, greatly enhancing the cellular repertoire of mRNA isoforms. As these isoforms can have altered stability, localisation and coding potential, deregulation of APA can disrupt gene expression and this has been linked to many diseases including cancer progression. How APA generates cancer-specific isoform profiles and what their physiological consequences are, however, is largely unclear. Here we use a sub...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Harry FischlJonathan NeveZhiqiao WangRadhika PatelAlastair LoueyBin TianAndre Furger
Reused by

Deep data QC

33/100 · F

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (Homo sapiens, Ion Torrent). Grade F, 33/100. Q30 critically low at 4.4% and mean quality 23.2 (both measured) fail standards for reliable expression quantification. Not recommended for reuse.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
Ion Torrent Proton
Platform
ION_TORRENT
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
32 / 32 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 31102657518 reported
total reads 261194362 reported
n content pct 0 measured
pct q20 bases 80.7 measured
pct q30 bases 4.4 measured
gc content pct 44.6 measured
mean read length 108.3 measured
mean base quality 23.2 measured
adapter content pct 0 measured
duplication rate pct 28.66 measured
supplementary file types BIGWIG, BW reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 33/100

The F grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 4.4 measured ×1 0%
mean base quality 23.2 measured ×0.6 0%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 28.66 measured ×0.4 100%
QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0