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mRNA-Seq profiling nephron progenitor and stromal progenitor cells in mouse embryonic kidney
To characterize the expression profile of mouse nephron progenitors, we performed intracellular labeling of Six2 by antibody, followed by FACS and RNA-seq. To characterize the expression profile of mouse stromal progenitor cells, we isolated Tdt+ cells from Foxd1Cre;Tdt transgenic mice kidney by FACS followed by RNA-seq.
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deposited / produced by
Albert D KimQiuyu GuoRiana ParvezNils LindstromAndrew McMahon
Deep data QC
metadata only · no data-level QC for this typeStandardized, field-standard QC computed by touching the data — every metric states how it was obtained
Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
19 / 19 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok
yes
reported
total bases
122925829612
reported
total reads
810325663
reported
supplementary file types
TXT
reported
QC cost
4 s compute
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0 · provisional — verify independently