Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE102888

GEO first seen 2018

Transcriptomic profile of the retinal ganglion cell type J-RGC

Organism
Mus musculus
Samples
2
Type
Expression profiling by high...
Submitted
2017-08-21

The goal of this experiment was to identify markers and potential regulators of J-RGC development.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Jinyue LiuJoshua R Sanes
Reused by

Deep data QC

90/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Bulk RNA-seq (mouse). Grade A: lower-than-peak base quality metrics (Q30=85.9%, mean Q=33.6) are the limiting factors, though still within acceptable ranges. Duplication (27.2%) is healthy, and modest N-content (0.028%) is acceptable. The 74.3 bp reads and moderate quality are suitable for standard differential expression but less ideal for sensitive isoform-level work.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.028 measured
pct q20 bases 92.6 measured
pct q30 bases 85.9 measured
gc content pct 41.9 measured
mean read length 74.3 measured
mean base quality 33.6 measured
adapter content pct 0.01 measured
duplication rate pct 27.22 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 90/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 85.9 measured ×1 80%
mean base quality 33.6 measured ×0.6 93%
adapter content pct 0.01 measured ×0.4 100%
duplication rate pct 27.22 measured ×0.4 100%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0