GSE102990

GEO first seen 2020

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Reused by

Single-cell lineage tracing by integrating CRISPR-Cas9 mutations... 2020 · 83 cites

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means

claude:haiku

Zebrafish RNA-seq with contradictory quality indicators. Exceptional base quality (Q30=97%, mean 35) is offset by unprecedented duplication (96.78%), suggesting either severe PCR amplification bias or ultra-depleted input RNA. Reusability is questionable; recommend investigating the source of extreme duplication before analysis, as it may violate abundance quantification assumptions.

Data type / assay

bulk-RNA-seq

Organism

Danio rerio

Metrics (value · how obtained)

n content pct 0.004 measured

pct q20 bases 97.7 measured

pct q30 bases 97 measured

gc content pct 62.6 measured

mean read length 76 measured

mean base quality 35 measured

adapter content pct 0 measured

duplication rate pct 96.78 measured

How this grade was computed

Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97 measured ×1 100%

mean base quality 35 measured ×0.6 100%

adapter content pct 0 measured ×0.4 100%

duplication rate pct 96.78 measured ×0.4 0%

QC cost 37 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0