Corpus 1,273 assessed · 1,174 scored · 643 reproduced ≥75 · 169 flagged ·∅ 74.1/100
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GSE103180

GEO first seen 2023

The Elongation Factor and Histone Chaperone Spt6 Maintains ESC Pluripotency by Controlling Super-Enhancers and Counteracting Polycomb Repressive Complex 2

Organism
Mus musculus
Samples
32
Type
Expression profiling by high...
Submitted
2017-08-28

Spt6 coordinates nucleosome dis- and re-assembly, transcriptional elongation and mRNA processing. Here, we report that depleting Spt6 in ESCs reduced expression of pluripotency factors, increased expression of cell lineage-affiliated developmental regulators, and induced cell morphological and biochemical changes indicative of ESC differentiation. Selective down-regulation of pluripotency factors upon Spt6 depletion may be mechanistically explained by its enrichment at ESC super-enhancers where...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
A H WangAster H JuanKyung D KoPei-Fang TsaiHossein ZareStefania Dell’OrsoVittorio Sartorelli
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
ChIP-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
32 / 32 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 57167943150 reported
total reads 1143358863 reported
n content pct 0.013 measured
pct q20 bases 99 measured
pct q30 bases 97 measured
gc content pct 40.6 measured
mean read length 50 measured
mean base quality 38.1 measured
adapter content pct 0.09 measured
duplication rate pct 5.23 measured
supplementary file types BED, DIFF reported
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 97 measured ×1 100%
QC cost 15 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0