Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE103202

GEO first seen 2018

Identification of genes regulated by long noncoding RNA H19 in skeletal muscle

Organism
Mus musculus
Samples
6
Type
Expression profiling by high...
Submitted
2017-08-28

Soleus muscle has the most abundant H19 expression compared to other skeletal muscle tissues. In order to identify genes regulated by long noncoding RNA H19 in skeletal muscle, we performed RNA-Seq with dissected WT and H19KO soleus muscles from 21 week old mice. Among the differentially expressed genes, we found skeletal muscle - overexpressed gene DUSP27, which potentially plays an important role in regulating skeletal muscle glucose metabolisim by regulating the activitiy of AMPK, might be a...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Na ZhangGordon G CarmichaelYingqun Huang
Reused by

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.006 measured
pct q20 bases 97.7 measured
pct q30 bases 96.6 measured
gc content pct 40 measured
mean read length 75.4 measured
mean base quality 35 measured
adapter content pct 0 measured
duplication rate pct 81.55 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.6 measured ×1 100%
mean base quality 35 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 81.55 measured ×0.4 0%
QC cost 32 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0