Corpus 1,285 assessed · 1,186 scored · 647 reproduced ≥75 · 174 flagged ·∅ 73.9/100
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GSE103528

GEO first seen 2018

CARM1/PRMT4 is essential for myeloid leukemogenesis but dispensable for normal hematopoiesis

Organism
Homo sapiens
Samples
27
Type
Expression profiling by high...
Submitted
2017-09-06

We investigated the role of PRMT4 in normal and malignant hematopoiesis. We found that knockdown of PRMT4 impairs cell cycle progression, induces apoptosis, and downgretulateds E2F target genes in leukemia cell lines, identifying several mechanisms for the leukemia-specific dependence on PRMT4.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Greenblatt SarahKarl Daniel
Reused by

Deep data QC

94/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human bulk RNA-seq. A-grade (94/100) with high base quality (Q30=96.1%) compensating for significant duplication (45.97%, scored 64/100), both measured. Duplication suggests PCR amplification bias affecting specific transcripts; findings should be validated with independent quantification methods. Fetch error prevented metadata retrieval but sequence metrics are fully measured.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.01 measured
pct q20 bases 97.5 measured
pct q30 bases 96.1 measured
gc content pct 47.1 measured
mean read length 75.5 measured
mean base quality 34.9 measured
adapter content pct 0 measured
duplication rate pct 45.97 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 94/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.1 measured ×1 100%
mean base quality 34.9 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 45.97 measured ×0.4 65%
QC cost 25 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0