Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE103568

GEO first seen 2018

Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq

Organism
Homo sapiens; Mus musculus
Samples
785
Type
Expression profiling by high...
Submitted
2017-09-06

Single-cell RNA sequencing (scRNA-seq) has emerged as a central genome-wide method to characterize cellular identities and processes. Consequently, improving its sensitivity, flexibility, and cost-efficiency can advance many research questions. Among the flexible plate-based methods, single-cell RNA barcoding and sequencing (SCRB-seq) is highly sensitive and efficient. Here, we systematically evaluate experimental conditions of this protocol and find that adding polyethylene glycol considerably...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Christoph ZiegenhainJohannes W Bagnoli
Reused by

Deep data QC

83/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Metrics (value · how obtained)
n content pct 0.003 measured
pct q20 bases 97.6 measured
pct q30 bases 94.3 measured
gc content pct 42.3 measured
mean read length 24 measured
mean base quality 35.1 measured
adapter content pct 0 measured
duplication rate pct 95.4 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 83/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 94.3 measured ×1 100%
mean base quality 35.1 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 95.4 measured ×0.4 0%
QC cost 24 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0