RNAseq on WT and Meteor KO Embryonic Stem Cells and day3 differentiated cells
We have demonstrated that Meteor KO cells are associated with a global transcriptional reprogramming associated to a block of Mesendoderm specification. Meteor KO cells loses their developmental competence for Mesendoderm specification in pluripotency and are redirected to a neuroectoderm fate.
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deep data QC
89/100 · BStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Mouse bulk RNA-seq (HiSeq 2500). Grade-B data (89/100) constrained by Q30=85.9% (scored 80/100) and adapter content 1.22% (incomplete removal), both measured. Base quality at this threshold still supports quantification but may compromise isoform-level resolution; acceptable for bulk differential analysis but not for sensitive applications.
The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0