GSE103695

Bicoid binding along anteroposterior axis of Drosophila Melanogaster embryos

Organism

Drosophila melanogaster; Drosophila pseudoobscura

Samples

2

Type

Genome binding/occupancy pro...

Submitted

2017-09-11

ChIP-Seq against Bicoid on manually dissected posterior thirds of Drosophila Melanogaster early stage 5 embryos.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by

Mustafa MirJenna HainesXiao-Yong LiMichael B EisenXavier Darzacq

Dense Bicoid hubs accentuate binding along the morphogen gradien... 2017

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay

ChIP-seq

Organism

Drosophila pseudoobscura; Drosophila melanogaster

Instrument

Illumina HiSeq 2500

Platform

ILLUMINA

Read type

short-read

Files available

FASTQ (raw reads)

N numbers (samples, groups)

2 / 2 runs

Completeness

100%

Metrics (value · how obtained)

checksum ok yes reported

total bases 11246206883 reported

total reads 111348583 reported

n content pct 0.012 measured

pct q20 bases 96.2 measured

pct q30 bases 93.6 measured

gc content pct 40.6 measured

mean read length 101 measured

mean base quality 37.4 measured

adapter content pct 2.51 measured

duplication rate pct 34.04 measured

supplementary file types WIG reported

How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published ChIP-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.6 measured ×1 100%

QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0