Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE103840

GEO first seen 2021

Single-cell RNA sequencing of sensory neurons in the mouse dorsal horn

Organism
Mus musculus
Samples
1,545
Type
Expression profiling by high...
Submitted
2017-09-13

We have used large-scale single-cell RNA sequencing (RNA-seq) to classify sensory neurons in the mouse dorsal horn, with the purpose of identifying neuronal subclasses, in particular making a systematic and comprehensive molecular classification of spinal cord sensory neurons, providing the neuronal basis for somatic sensation.

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Martin HäringAmit ZeiselSten LinnarssonPatrik Ernfors
Reused by

Deep data QC

80/100 · B

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Large-scale Illumina HiSeq 2000 RNA-seq of Mus musculus yields 57.0 billion bases across 1.1 billion reads at 95.5% Q20 with geo_uid confirmed in NCBI. Extensive dataset with verified provenance; comprehensive coverage enables genome-wide expression profiling and splicing-variant discovery.

Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2000
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
2,157 / 1,545 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 56969833529 reported
total reads 1148918514 reported
n content pct 0 measured
pct q20 bases 95.5 measured
pct q30 bases 90.6 measured
gc content pct 41.1 measured
mean read length 51 measured
mean base quality 35.7 measured
adapter content pct 13.34 measured
duplication rate pct 56.95 measured
supplementary file types XLSX reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 80/100

The B grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 90.6 measured ×1 100%
mean base quality 35.7 measured ×0.6 100%
adapter content pct 13.34 measured ×0.4 37%
duplication rate pct 56.95 measured ×0.4 40%
QC cost 12 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0