Corpus 1,272 assessed · 1,173 scored · 643 reproduced ≥75 · 168 flagged ·∅ 74.1/100
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GSE103939

GEO first seen 2018

DNA methylation profiling of Daphnia species.

Organism
Daphnia pulex; Daphnia magna
Samples
41
Type
Methylation profiling by hig...
Submitted
2017-09-18

To profile the Daphnia species methylome and to achieve a better understanding of the level of variations in the methylome of Daphnia species, we performed whole genome bisulfite sequencing (WGBSeq) of adult Daphnia magna Bham2 strain and Daphnia pulex Eloise Butler strain (EB45 and EB31 strains). We also analysed the correlation between gene expression and methylation in the two species, using data generated in this study and RNA-seq data from Orsini, et al. 2016. We found that methylation perc...

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Jouni KvistCamila Gonçalves AthanásioLeda Mirbahai
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

insufficient data to score

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained

Data type / assay
methylation
Organism
Daphnia magna
Metrics (value · how obtained)
n content pct 0 measured
pct q20 bases 97.5 measured
pct q30 bases 93.2 measured
gc content pct 28.5 measured
mean read length 151 measured
mean base quality 35.7 measured
adapter content pct 36.7 measured
duplication rate pct 3.81 measured
How this grade was computed

The insufficient grade is a transparent weighted average. Each metric below scored from 0–100% against the published methylation thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.2 measured ×1 100%
QC cost 30 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0