Impeding transcription of expanded microsatellite repeats by deactivated Cas9
Transcription of expanded microsatellite repeats is associated with multiple human diseases, including myotonic dystrophy, Fuchs’ endothelial corneal dystrophy, and C9orf72-ALS/FTD. Eliminating or reducing production of RNA and proteins arising from these expanded loci holds therapeutic benefit. Here, we tested the hypothesis that a deactivated form of the Cas9 enzyme impedes transcription across expanded microsatellites. We observed a repeat length-, PAM-, and strand-dependent reduction in the...
Provenance — who produced it, who reused it
Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
- Impeding Transcription of Expanded Microsatellite Repeats by Dea... 2017 · 135 cites
Deep data QC
93/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Human bulk RNA-seq with provisional A grade (93/100) and incomplete data validity: a fetch error prevented full metric inspection. Significant duplication bias (49.83%, scored 56/100) is the primary concern, suggesting PCR over-amplification. Deep measured revalidation is pending before confident reuse in quantitative expression work.
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0