Corpus 1,277 assessed · 1,178 scored · 644 reproduced ≥75 · 170 flagged ·∅ 74/100
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GSE104295

GEO first seen 2017

Analysis of gene expression in SKOV3 ovarian cancer cells after knockdown of the long non-coding RNA DNM3OS

Organism
Homo sapiens
Samples
6
Type
Expression profiling by high...
Submitted
2017-09-26

RNA-sequencing was performed to gain insight into the mechanism responsible for the mesenchymal-to-epithelial transition (MET) induced by loss of long non-coding RNA (lncRNA) DNM3OS in SKOV3 ovarian cancer cells. Following siRNA-mediated knockdown of DNM3OS or non-targeting control, RNA-sequencing was performed. This high-throughput data revealed knockdown of DNM3OS down-regulated the expression of genes and pathways known to induce EMT in ovarian cancer.

Provenance — who produced it, who reused it

Linked to 2 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
Christine M Eischen
Reused by

1 further paper cites this accession but reuse could not be confirmed.

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Metrics (value · how obtained)
n content pct 0.018 measured
pct q20 bases 95.6 measured
pct q30 bases 93.6 measured
gc content pct 48.8 measured
mean read length 75.5 measured
mean base quality 34.3 measured
adapter content pct 0 measured
duplication rate pct 20.32 measured
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 93.6 measured ×1 100%
mean base quality 34.3 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 20.32 measured ×0.4 100%
QC cost 26 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0