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A broad single-cell transcriptome view of the male mouse germ line
Single-cell RNAseq of 2550 cells from the testes of two mice.
Provenance — who produced it, who reused it
Linked to 4 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.
Deposited / produced by
Andreas WinterpachtSoeren Lukassen
Reused by
- Characterization of germ cell differentiation in the male mouse... 2018 · 102 cites
3 further papers cite this accession but reuse could not be confirmed.
Deep data QC
100/100 · AStandardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured
Data type / assay
bulk-RNA-seq
Organism
Mus musculus
Instrument
Illumina HiSeq 2500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
2 / 2 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok
yes
reported
total bases
49310854692
reported
total reads
397668183
reported
n content pct
0.005
measured
pct q20 bases
98.8
measured
pct q30 bases
97.1
measured
gc content pct
50.4
measured
mean read length
26
measured
mean base quality
36.5
measured
adapter content pct
0
measured
duplication rate pct
0.65
measured
supplementary file types
MTX, TSV
reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100
The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.
pct q30 bases
97.1
measured
×1
100%
mean base quality
36.5
measured
×0.6
100%
adapter content pct
0
measured
×0.4
100%
duplication rate pct
0.65
measured
×0.4
100%
QC cost
16 s compute
measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0