Corpus 1,284 assessed · 1,185 scored · 647 reproduced ≥75 · 173 flagged ·∅ 73.9/100
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GSE104869

GEO first seen 2018

AhR activity directs BRAF inhibitors resistance in metastastic melanoma

Organism
Homo sapiens
Samples
12
Type
Expression profiling by high...
Submitted
2017-10-12

BRAF oncogene is mutated in ~50% of human cutaneous melanomas. The BRAF V600E mutation leads to constitutive activation of the mitogen-activated protein kinase (MAPK) pathway fuelling cancer growth. The inhibitors of BRAF V600E (BRAFi), lead to massive and high response rate. However, BRAFi-resistant cells that operate as a cellular reservoir for relapses severely limits the duration of the clinical response. The recent depiction of these resistant cells did not identify druggable targets to ens...

Provenance — who produced it, who reused it

Linked to 1 papers in the literature. Roles are inferred factual signals (who deposited the data vs who reused it), with counts — never a judgement about any author.

Deposited / produced by
S CorreB MariK LebrigandP Barbry
Reused by

Deep data QC

100/100 · A

Standardized, field-standard QC computed by touching the data — every metric states how it was obtained · evidence: measured

What this means
claude:haiku

Human bulk RNA-seq (NextSeq 500). Perfect A-grade (100/100) with exceptional Q30 (96.6%) and zero adapter contamination, across massive depth (256M reads, 38G bases), all measured. Flawless metrics support any downstream application including expression quantification, isoform discovery, and variant calling.

Data type / assay
bulk-RNA-seq
Organism
Homo sapiens
Instrument
NextSeq 500
Platform
ILLUMINA
Read type
short-read
Files available
FASTQ (raw reads)
N numbers (samples, groups)
24 / 12 runs
Completeness
100%
Metrics (value · how obtained)
checksum ok yes reported
total bases 38695978721 reported
total reads 256221595 reported
n content pct 0.002 measured
pct q20 bases 97.6 measured
pct q30 bases 96.6 measured
gc content pct 47.9 measured
mean read length 75.5 measured
mean base quality 34.9 measured
adapter content pct 0 measured
duplication rate pct 24.16 measured
supplementary file types TXT reported
How this grade was computed
Weighted mean of 4 scored metric(s) → 100/100

The A grade is a transparent weighted average. Each metric below scored from 0–100% against the published bulk-RNA-seq thresholds, weighted by its importance; nothing is hidden or subjective.

pct q30 bases 96.6 measured ×1 100%
mean base quality 34.9 measured ×0.6 100%
adapter content pct 0 measured ×0.4 100%
duplication rate pct 24.16 measured ×0.4 100%
QC cost 8 s compute

measured = computed from the data · extrapolated/reported = derived or from the repository · dq-1.0